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标题:Taxonomic annotation of 16S rRNA sequences of pig intestinal samples using MG-RAST and QIIME2 generated different microbiota compositions
时间:2021-08-06 15:43:38
DOI:10.1016/j.mimet.2021.106235
大小:4548 kb
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目录:
  • Make microalgal cultures axenic again – a fast and simple workflow utilizing fluorescence-activated cell sorting
    • 1 Introduction
    • 2 Materials and methods
      • 2.1 Microalgae strains
      • 2.2 Cultivation parameters
      • 2.3 Axenicity check
      • 2.4 Fluorescence-activated cell sorting (FACS)
    • 3 Results and discussion
      • 3.1 Method development
        • 3.1.1 Gate definition and FACS
        • 3.1.2 Plate spreading and cell isolation
      • 3.2 Green algae
      • 3.3 Method applicability to different FACS devices
    • 4 Conclusion
    • Statement of informed consent
    • Declaration of Competing Interest
    • Acknowledgments
    • References

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