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标题:Rational design of medium supplementation strategy for improved influenza viruses production based on analyzing nutritional requirements of MDCK Cells
时间:2021-08-01 10:41:55
DOI:10.1016/j.vaccine.2014.10.067
大小:1721 kb
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目录:
  • Rational design of medium supplementation strategy for improved influenza viruses production based on analyzing nutritiona...
    • 1 Introduction
    • 2 Materials and methods
      • 2.1 Cell lines and culture conditions
      • 2.2 Cell counting
      • 2.3 Infection of MDCK cells
      • 2.4 Determination of virus titers
      • 2.5 Quantification of metabolites
    • 3 Results
      • 3.1 Effects of medium-exchanging on cell viability and influenza virus production
      • 3.2 Effects of influenza virus infection on cell death and metabolism
        • 3.2.1 Growth and death of MDCK cells upon virus infection
        • 3.2.2 Metabolism of MDCK cells
      • 3.3 Effects of glucose and amino acids concentration on MDCK cell death and influenza virus production
      • 3.4 MDCK cell growth and influenza virus production in nutritional adjusted MM
    • 4 Discussion
    • Acknowledgements
    • Appendix A Supplementary data
    • References

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