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标题:Preparation and characterization of chitosan–carbon nanotube scaffolds for bone tissue engineering
时间:2021-07-31 09:55:06
DOI:10.1016/j.ijbiomac.2011.12.032
大小:2667 kb
页数:11 PAGES
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目录:
  • Preparation and characterization of chitosan–carbon nanotube scaffolds for bone tissue engineering
    • 1 Introduction
    • 2 Materials and methods
      • 2.1 Functionalization and purification of carbon nanotubes (CNTs)
      • 2.2 Preparation of chitosan and chitosan–multiwalled carbon nanotube scaffold
        • 2.2.1 Porosity measurement
        • 2.2.2 Water uptake and retention abilities
        • 2.2.3 Thermogravimmetric analysis
        • 2.2.4 FTIR spectrometry
        • 2.2.5 X-ray diffraction analysis
        • 2.2.6 Scanning electron microscopy and optical microscopy analysis
        • 2.2.7 Cell culture
        • 2.2.8 In vitro cytotoxicity and cell proliferation assay
        • 2.2.9 Hoechst 33342 staining
        • 2.2.10 Total protein measurement
        • 2.2.11 Alkaline phosphatase activity
        • 2.2.12 Mineralization assay
        • 2.2.13 Statistical analysis
    • 3 Results and discussion
      • 3.1 Material selection and scaffold preparation
      • 3.2 Gross examination of the scaffolds
      • 3.3 Porosity
      • 3.4 Water uptake and retention abilities
      • 3.5 Stretching frequency of the scaffolds
      • 3.6 Thermal stability of the scaffolds
      • 3.7 X-ray diffraction studies
      • 3.8 Morphology studies of the scaffolds
      • 3.9 Hoechst 33342 staining for MG-63 live cell
      • 3.10 In vitro analysis of scaffold with osteoblast like cells
    • 4 Conclusion
    • Acknowledgement
    • References

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