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标题:Forced expression of HOXA13 confers oncogenic hallmarks to esophageal keratinocytes
时间:2021-07-28 13:52:19
DOI:10.1016/j.bbadis.2020.165776
PMID:32222541
大小:8392 kb
页数:13 PAGES
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目录:
  • Forced expression of HOXA13 confers oncogenic hallmarks to esophageal keratinocytes
    • Introduction
    • Methods
      • Cell line
      • Generation of EPC2-hTERT HOXA13 overexpression model
      • RNA isolation
      • RNA-Seq
      • FACS
      • MTT assay
      • Cell adhesion test
      • 3D culture EPC2-hTERT cells
      • Histology and immunohistochemistry of 3D culture
      • 2D Migration assays
      • 3D-migration using cell dispersion assay
      • Phosphoprotein profiling
      • Drug sensitivity assay
      • Statistics
    • Results
      • HOXA13 alters keratinocyte gene expression profiles
      • HOXA13 influences oncogenic cellular phosphoprofile
      • HOXA13 downregulates MHC class I in keratinocytes
      • HOXA13 overexpression provides a proliferative advantage to esophageal keratinocytes and decreases paclitaxel-induced cell death
      • HOXA13 overexpression changes the morphology and differentiation status of EPC2-hTERT-derived spheroids
      • HOXA13 promotes cellular migration
    • Discussion
    • Conclusions
    • CRediT authorship contribution statement
    • Declaration of competing interest
    • Acknowledgements
    • References

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