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标题:Glutathione Modulates Ca2+ Influx and Oxidative Toxicity Through TRPM2 Channel in Rat Dorsal Root Ganglion Neurons
时间:2021-07-28 11:59:46
DOI:10.1007/s00232-011-9382-6
大小:523 kb
页数:11 PAGES
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目录:
  • Glutathione Modulates Ca2+ Influx and Oxidative Toxicity Through TRPM2 Channel in Rat Dorsal Root Ganglion Neurons
    • Abstract
    • Materials and Methods
      • Preparation of Cell Samples
      • Electrophysiology
      • Study Groups for Oxidant/Antioxidant and Cytosolic Ca2+ Release Analyses in DRG Neurons
      • Measurement of Intracellular Free Calcium Concentration ([Ca2+]i)
      • Lipid Peroxidation (LP), Reduced Glutathione (GSH), Glutathione Peroxidase (GSH-Px), and Protein Assay
      • Statistical Analyses
    • Results
      • Effects of GSH on TRPM2 Channels Activated by BSO
      • Effects of Intracellular GSH on TRPM2 Channels Activated by BSO
      • Effects of TRPM2 Antagonist in DRG Neurons
      • Effects of Extracellular GSH and 2-APB on [Ca2+]i Release in DRG Neurons
      • Effects of Extracellular GSH and 2-APB on GSH-Px, GSH, and Lipid Peroxidation Values in DRG Neurons
    • Discussion
    • Acknowledgments
    • References

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