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标题:FAM172A Deletion May Enhance Hepatic Steatosis by Promoting ER Stress
时间:2021-07-28 11:06:08
DOI:10.1007/s10620-020-06601-y
PMID:32945983
大小:6017 kb
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目录:
  • FAM172A Deletion May Enhance Hepatic Steatosis by Promoting ER Stress
    • Abstract
      • Background
      • Aims
      • Methods
      • Results
      • Conclusions
    • Introduction
    • Materials and Methods
      • Reagents and Antibodies
      • Animal Studies
      • Biochemical and Histological Analyses
      • Isolation of Primary Hepatocytes
      • Cell Culture and Treatment
      • Construction of Plasmids and Transient Transfection
      • Western Blotting
      • Measurement of Triglyceride Levels
      • Determination of Intracellular FAM172A Location
      • Immunofluorescence
      • Immunocoprecipitation
      • Cell Viability Assay
      • Caspase-37 Activity Assay
      • Quantitative Real-Time Reverse Transcription PCR (qRT-PCR)
      • Statistical Analysis
    • Results
      • The Lack of FAM172A Promotes Steatosis in a Mouse Model of NAFLD
      • FAM172A Knockdown Aggravates Liver Injury in a Mouse Model of ER Stress
      • FAM172A Inhibited ER Stress Through IRE1α, ATF6, and eIF2a Pathways
      • FAM172A−− Cells are Sensitive to ER Stress-Induced Apoptosis and Inflammation
    • Discussion
    • References

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