| 标题： | Pathogenic Huntingtin Repeat Expansions in Patients with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis |
| 时间： | 2021-04-07 20:48:47 |
| DOI： | 10.1016/j.neuron.2020.11.005 |
| 大小： | 1002 kb |
| 页数： | 11 PAGES |
| 下载： | 点击下载 |
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目录：
- Metabolic engineering of Saccharomyces cerevisiae for bioconversion of d-xylose to d-xylonate
- Introduction
- Materials and Methods
- Strains and strain construction
- Media and culture conditions
- Measurement of biomass
- Chemical analyses
- Determination of vitality and viability of d-xylonate producing cells
- Enzyme purification
- Enzyme activity
- NMR analyses
- In situ pHluorin calibration and measurement
- Results
- d-xylose dehydrogenases and their activity in recombinant S. cerevisiae strains
- Production of d-xylonate with the d-xylose dehydrogenase XylB
- Effect of d-xylonolactone lactonase XylC on production of d-xylonate
- Effect of d-xylonate production on cellular physiology
- Discussion
- Acknowledgments
- References
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