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标题:Determination of oroxylin A and oroxylin A 7-O-d-glucuronide in HepG2 cell lysate and subcellular fractions with SPE-UPLC–MS/MS: Cellular pharmacokinetic study to indicate anti-cancer mechanisms
时间:2021-03-22 20:30:26
DOI:10.1016/j.jpba.2018.03.019
PMID:29571134
大小:1512 kb
页数:10 PAGES
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目录:
  • Determination of oroxylin A and oroxylin A 7-O-d-glucuronide in HepG2 cell lysate and subcellular fractions with SPE-UPLC–...
    • 1 Introduction
    • 2 Material and methods
      • 2.1 Material and reagents
      • 2.2 Cell culture
      • 2.3 Preparation of cell sample and subcellular fractions
      • 2.4 Preparation of samples for UPLC–MS/MS
      • 2.5 Instrumental conditions for UPLC–MS/MS analysis
      • 2.6 Standard solutions and method validation
        • 2.6.1 Preparation of stock and working solutions
        • 2.6.2 Specificity and carry-over
        • 2.6.3 Calibration standards and quality control samples
        • 2.6.4 Accuracy and precision
        • 2.6.5 The absolute recovery and matrix effect
        • 2.6.6 The impact of isolation procedure on subcellular concentration of OA and OG and the recovery of post SPE preparation
        • 2.6.7 Stability
      • 2.7 Data analysis
    • 3 Results and discussion
      • 3.1 Method development
        • 3.1.1 MS/MS optimization
        • 3.1.2 Chromatographic optimization
        • 3.1.3 Specificity and selectivity
        • 3.1.4 Precision and accuracy
        • 3.1.5 Matrix effect and absolute recovery
        • 3.1.6 Stability
      • 3.2 Determination of OA and OG in the whole cell and subcellular fractions by SPE-UPLC–MS/MS
    • 4 Conclusion
    • Conflict of interest
    • Acknowledgement
    • Appendix A Supplementary data
    • References

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