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标题:TRIM8 interacts with KIF11 and KIFC1 and controls bipolar spindle formation and chromosomal stability
时间:2021-02-23 22:19:41
DOI:10.1016/j.canlet.2019.12.042
大小:1878 kb
页数:10 PAGES
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目录:
  • TRIM8 interacts with KIF11 and KIFC1 and controls bipolar spindle formation and chromosomal stability
    • Introduction
    • Material and methods
      • Cell lines and infection
      • Expression plasmids
      • Immunoprecipitation and mass spectrometry analysis
      • Co-immunoprecipitation and Western blot
      • Silencing experiments
      • RNA isolation and quantitative PCR (qPCR)
      • Fluorescence and confocal microscopy
      • Live-cell imaging
      • Cell synchronization
      • Flow cytometric analysis
      • Metaphase spreads and karyotyping
    • Results
      • TRIM8 physically interacts with mitotic proteins
      • Disentangling TRIM8 in mitotic spindle processes
      • TRIM8 silencing slows down mitosis progression
      • TRIM8 is required for chromosomal stability
    • Discussion
    • Author contribution
    • mk:H1_21
    • Acknowledgments
    • Supplementary data
    • References

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