在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:Hypoxic mesenchymal stem cell-derived exosomes promote bone fracture healing by the transfer of miR-126
时间:2021-02-23 18:24:57
DOI:10.1016/j.actbio.2019.12.020
大小:5729 kb
页数:18 PAGES
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

目录:
  • Hypoxic mesenchymal stem cell-derived exosomes promote bone fracture healing by the transfer of miR-126
    • 1 Introduction
    • 2 Materials and methods
      • 2.1 Cell culture and hypoxia treatment
      • 2.2 Exosome isolation and identification
      • 2.3 Exosome uptake by HUVECs
      • 2.4 Femoral fracture model and X-ray imaging
      • 2.5 Microcomputed tomography imaging (micro-CT)
      • 2.6 Micro-CT analysis of angiogenesis at the fracture sites
      • 2.7 Immunofluorescence staining
      • 2.8 MiRNA/siRNA transfection
      • 2.9 Real-time RT-PCR
      • 2.10 Exosomal miRNA microarray assay
      • 2.11 Cell counting Kit-8 (CCK8) assay
      • 2.12 5-Ethynyl-2′-deoxyuridine (EDU) assay
      • 2.13 Tube formation assay
      • 2.14 Migration assay
      • 2.15 Luciferase reporter assay
      • 2.16 Chromatin immunoprecipitation (CHIP) assay
      • 2.17 Western blot analysis
      • 2.18 Statistical analysis
    • 3 Results
      • 3.1 Identification of HucMSCs
      • 3.2 Hypoxia promotes exosome release from HucMSCs
      • 3.3 The differential uptake of exosomes by HUVECs is dependent upon oxygen status
      • 3.4 Bone fracture healing is promoted in mice after transplantation with Hypo-Exos
      • 3.5 Hypo-Exos promote proliferation, migration, and tube formation in HUVECs
      • 3.6 The miR-126 is upregulated in Hypo-Exos and transferred to HUVECs by exosomes
      • 3.7 Knockdown of miR-126 inhibits Hypo-Exos-mediated proliferation, angiogenesis and migration in vitro and in vivo
      • 3.8 Exosomal miR-126 regulates SPRED1 by directly targeting the 3′-UTR
      • 3.9 Exosomal miR-126 promotes HUVECs proliferation, angiogenesis and migration by targeting SPRED1
      • 3.10 HIF-1α induces the enrichment of miR-126 in exosomes shed from hypoxic HucMSCs
      • 3.11 Exosomal miR-126 promotes proliferation, angiogenesis and migration in HUVECs via the SPRED1/Ras/Erk pathway
    • 4 Discussion
    • 5 Conclusions
    • Declaration of Competing Interest
    • Acknowledgements
    • Supplementary materials
    • References

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系