| 标题： | How formaldehyde reacts with amino acids |
| 时间： | 2021-02-23 14:45:56 |
| DOI： | 10.1038/s42004-019-0224-2 |
| 大小： | 1005 kb |
| 页数： | 15 PAGES |
| 下载： | 点击下载 |
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目录：
- How formaldehyde reacts with amino acids
- Results
- Product identification in reactions of AAs and HCHO
- pH and concentration dependence studies
- Assessing product stabilities
- Competition between Cys and different AAs for reaction with HCHO
- Comparison of the reactivity of Cys with HCHO using different electrophiles
- Reductive Nα-methylation
- Discussion
- Methods
- General methods
- Standard conditions reaction setup
- Adjusted pH reactions
- pH dependent equilibria experiments
- Stability assays using 1,3-cyclohexadione
- Lyophilisation to test stability of HCHO adducts
- Using 13C labelled HCHO to test for exchange of HCHO adducts
- Competition assays
- Spectator AA experiments
- Reductive amination
- Product characterisation
- Cysteine products
- Homocysteine products
- Penicillamine products
- Serine product
- Threonine product
- allo-Threonine product
- Homoserine product
- Asparagine products
- Glutamine product
- Histidine products
- Tryptophan products
- Arginine products
- Ornithine products
- Lysine product
- Cysteine and acetaldehyde products
- Cysteine and acetone product
- Cysteine and glyoxal products
- Cysteine and methylglyoxal products
- Data availability
- References
- Acknowledgements
- Author contributions
- Competing interests
- Additional information
- Results
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