在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:Activation of a putative MAP kinase in pollen is stimulated by the self-incompatibility (SI) response
时间:2021-02-23 12:00:06
DOI:10.1016/s0014-5793(03)00710-5
大小:265 kb
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

目录:
  • Activation of a putative MAP kinase in pollen is stimulated by the self-incompatibility (SI) response
    • Introduction
    • Materials and methods
      • Plant material and pollen treatments
      • MBP in-gel MAPK assay
      • Immunoprecipitation with anti-phosphotyrosine antiserum
      • Immunoblotting
    • Results
      • Activation of a 56 kDa pollen MBP kinase during the SI response
      • p56 activity is immunoprecipitated with anti-phosphotyrosine antibody
      • p56 activity is detected by antibodies recognising activated MAPKs
      • Activated p56 is sensitive to the MAPK inhibitor apigenin
      • p56 activity is stimulated by mastoparan
      • p56 activity requires influx of extracellular Ca2+
    • Discussion
      • SI-specific enhanced activation of a pollen MBP kinase
      • Is p56 activation involved in regulation of pollen tube tip growth?
    • Acknowledgements
    • References

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系