| 标题： | LncRNA MAGI2-AS3 inhibits the self-renewal of leukaemic stem cells by promoting TET2-dependent DNA demethylation of the LRIG1 promoter in acute myeloid leukaemia |
| 时间： | 2021-02-21 19:34:18 |
| DOI： | 10.1080/15476286.2020.1726637 |
| 大小： | 3326 kb |
| 页数： | 12 PAGES |
| 下载： | 点击下载 |
| 预览： |
目录：
- Abstract
- Introduction
- Results
- Downregulation of lncRNA MAGI2-AS3 in LSCs
- LncRNA MAGI2-AS3 inhibits the LSCs self-renewal
- LncRNA MAGI2-AS3 inhibits the LSCs self-renewal by promoting LRIG1 expression
- LRIG1 promoter region is methylated in AML
- LncRNA MAGI2-AS3 recruits TET2 to the LRIG1 promoter region and causes DNA demethylation of LRIG1
- LncRNA MAGI2-AS3 inhibits the LSCs self-renewal by promoting TET2-dependent DNA demethylation of the LRIG1 promoter
- MAGI2-AS3 inhibits the LSCs self-renewal and increases survival rate of NOD/SCID mice with AML invivo
- Discussion
- Materials and methods
- Ethics statement
- Samples
- Isolation and sorting of leukaemia stem cells (LSCs)
- Fluorescence insitu hybridization (FISH) assay
- Cell treatment
- RNA extraction and quantification
- Western blot analysis
- Sphere formation and colony formation assay
- Fractionation of nuclear and cytoplasmic RNA
- Methylation-specific PCR (MS-PCR)
- RNA pull-down
- RIP assay
- Chromatin immunoprecipitation (ChiP) assay
- Animal experiment invivo
- Statistical analysis
- Disclosure statement
- Funding
- References
本页内容由网络收集而来，版权归原创者所有，如有侵权请及时联系