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标题:LncRNA MAGI2-AS3 inhibits the self-renewal of leukaemic stem cells by promoting TET2-dependent DNA demethylation of the LRIG1 promoter in acute myeloid leukaemia
时间:2021-02-21 19:34:18
DOI:10.1080/15476286.2020.1726637
大小:3326 kb
页数:12 PAGES
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目录:
  • Abstract
  • Introduction
  • Results
    • Downregulation of lncRNA MAGI2-AS3 in LSCs
    • LncRNA MAGI2-AS3 inhibits the LSCs self-renewal
    • LncRNA MAGI2-AS3 inhibits the LSCs self-renewal by promoting LRIG1 expression
    • LRIG1 promoter region is methylated in AML
    • LncRNA MAGI2-AS3 recruits TET2 to the LRIG1 promoter region and causes DNA demethylation of LRIG1
    • LncRNA MAGI2-AS3 inhibits the LSCs self-renewal by promoting TET2-dependent DNA demethylation of the LRIG1 promoter
    • MAGI2-AS3 inhibits the LSCs self-renewal and increases survival rate of NOD/SCID mice with AML invivo
  • Discussion
  • Materials and methods
    • Ethics statement
    • Samples
    • Isolation and sorting of leukaemia stem cells (LSCs)
    • Fluorescence insitu hybridization (FISH) assay
    • Cell treatment
    • RNA extraction and quantification
    • Western blot analysis
    • Sphere formation and colony formation assay
    • Fractionation of nuclear and cytoplasmic RNA
    • Methylation-specific PCR (MS-PCR)
    • RNA pull-down
    • RIP assay
    • Chromatin immunoprecipitation (ChiP) assay
    • Animal experiment invivo
    • Statistical analysis
  • Disclosure statement
  • Funding
  • References

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