| 标题： | Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq |
| 时间： | 2021-01-14 13:47:30 |
| DOI： | 10.1007/978-1-4939-3067-8_13 |
| 大小： | 293 kb |
| 页数： | 19 PAGES |
| 下载： | 点击下载 |
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目录：
- Chapter 13: Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq
- 1 Introduction
- 2 Materials
- 2.1 Transfection and miRNA Mimics
- 2.2 Bead Preparation and Pulldown
- 2.3 RNA Precipitation and Cleanup
- 2.4 RNA-seq Library Preparation
- 2.5 Real-Time PCR
- 2.6 MRE Validation
- 3 Methods
- 3.1 Transfection
- 3.2 Bead Preparation
- 3.3 Cell Lysis and Pulldown Incubation
- 3.4 Bead Washing and RNA Precipitation
- 3.5 RNA Cleanup
- 3.6 Pulldown Analysis
- 3.7 Pulldown-seq Library Prep and Analysis
- 3.8 Target Validation
- 3.9 RNase Treatment for Identification of MREs
- 3.10 IMPACT-seq Library Prep and Analysis
- 3.11 Analysis and Validation of MREs
- 3.12 Bioinformatics Analysis of Target Genes
- 4 Notes
- References
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