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标题:Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq
时间:2021-01-14 13:47:30
DOI:10.1007/978-1-4939-3067-8_13
大小:293 kb
页数:19 PAGES
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目录:
  • Chapter 13: Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq
    • 1 Introduction
    • 2 Materials
      • 2.1 Transfection and miRNA Mimics
      • 2.2 Bead Preparation and Pulldown
      • 2.3 RNA Precipitation and Cleanup
      • 2.4 RNA-seq Library Preparation
      • 2.5 Real-Time PCR
      • 2.6 MRE Validation
    • 3 Methods
      • 3.1 Transfection
      • 3.2 Bead Preparation
      • 3.3 Cell Lysis and Pulldown Incubation
      • 3.4 Bead Washing and RNA Precipitation
      • 3.5 RNA Cleanup
      • 3.6 Pulldown Analysis
      • 3.7 Pulldown-seq Library Prep and Analysis
      • 3.8 Target Validation
      • 3.9 RNase Treatment for Identification of MREs
      • 3.10 IMPACT-seq Library Prep and Analysis
      • 3.11 Analysis and Validation of MREs
      • 3.12 Bioinformatics Analysis of Target Genes
    • 4 Notes
    • References

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