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标题:Hyperhomocysteinemia-induced death of retinal ganglion cells: The role of Müller glial cells and NRF2
时间:2020-11-22 21:56:48
DOI:10.1016/j.redox.2019.101199
PMID:31026769
大小:3422 kb
页数:14 PAGES
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目录:
  • Hyperhomocysteinemia-induced death of retinal ganglion cells: The role of Müller glial cells and NRF2
    • Introduction
    • Methods and materials
      • Animals
      • Evaluation of retinal function and structure
        • Optical coherence tomography (OCT)
        • Electroretinography
        • Visual acuity assessment
        • Intraocular pressure
        • Histologic processing of tissue and microscopic analysis
      • Cell culture studies
        • Isolation and culture of mouse Müller cells
        • Co-culture of primary RGCs with primary Müller cells
        • Hcy treatment of cultured cells
        • Assessment of cell survival and cell death
      • Glycolysis and mitochondrial function assays
      • Data analysis
    • Results
      • Effects of acute Hhcy exposure assessed using SD-OCT imaging
      • Absence of NRF2 reduces inner retinal thickness in Hhcy models
      • Absence of NRF2 attenuates visual acuity in Hhcy mouse models
      • Absence of NRF2 accelerates the loss of GCL cells in Hhcy mouse models
      • Absence of NRF2 increases GFAP levels in Hhcy mouse models
      • Müller glial-specific Nrf2 is critical for RGC survival under conditions of elevated Hcy: in vitro indirect co-culture studies
      • Hcy increases the spare respiratory capacity and glycolytic reserve in WT Müller cells, but not in Nrf2−/− Müller cells
    • Discussion
    • Conflicts of interest
    • Acknowledgements
    • Supplementary data
    • References

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