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标题:Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes
时间:2020-11-22 18:58:46
DOI:10.1016/j.bmc.2015.08.041
大小:1799 kb
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目录:
  • Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes
    • 1 Introduction
    • 2 Results and discussion
      • 2.1 Synthesis
      • 2.2 qPCR studies
    • 3 Conclusions
    • 4 Experimental
      • 4.1 N-\(9-\(2,5-Dicarboxyphenyl\)-6-\(ethylamino\)-2,7-dimethyl-3H-xanthen-3-ylidene\) ethanaminium chloride \(6-carboxyrho- damine-6G\) \(1\)
      • 4.2 3′-\(N-Ethyl-2,2,2-trifluoroacetamido\)-6′-\(ethylamino\)-2′,7′-dimethyl-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carbo- xylic acid \(3′,6′-N-ditrifluoroacetyl-6-carboxyrhodamine-6G\) \�
        • 4.2.1 Method A
        • 4.2.2 Method B
      • 4.3 N,N′-\(6-\(\(6-Hydroxyhexyl\)carbamoyl\)-2′,7′-dimethyl-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl\)-bis\(N-ethyl-2, 2,2-trifluoroacetamide\) \(6-\(3′,6′-N-ditrifluoroacety
      • 4.4 \(6-\(3′-\(N-Ethyl-2,2,2-trifluoroacetamido\)-6′-\(ethylamino\)-2′,7′-dimethyl-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-6-carboxamido\)hexyl\)-2-cyanoethyl-diisopropylphosphoramidite \(6-\(3′,6
      • 4.5 N,N′-\(6-\(\(3-Azidopropyl\)carbamoyl\)-2′,7′-dimethyl-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-3′,6′-diyl\)-bis\(N-ethyl-2,2,2-trifluoroacetamide\) \(6-\(\(3-azidopropyl\)carbamoyl\)-\(3&prime�
      • 4.6 N-\(9-\(5-\(\(3-Azidopropyl\)carbamoyl\)-2-carboxyphenyl\)-6-\(ethylamino\)-2,7-dimethyl-3H-xanthen-3-ylidene\)ethanamin- ium chloride \(6-\(\(3-azidopropyl\)carbamoylrhodamine 6G\) \(6\)
      • 4.7 Oligonucleotide synthesis and purification
      • 4.8 PCR
    • Acknowledgements
    • Supplementary data
    • References and notes

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