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标题:Influence of short-term exposure to high light on photosynthesis and proteins involved in photo-protective processes in tomato leaves
时间:2020-11-22 17:00:27
DOI:10.1016/j.envexpbot.2020.104198
大小:2393 kb
页数:15 PAGES
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目录:
  • Influence of short-term exposure to high light on photosynthesis and proteins involved in photo-protective processes in tomato leaves
    • Introduction
    • Materials and methods
      • Plant material and growth conditions
      • Pigment and flavonoid content
      • Chlorophyll-a fluorescence
      • Leaf proteomics using shotgun approach
        • Sample preparation
        • Liquid chromatography and mass spectrometry
        • Protein identification
        • Data processing and bioinformatics analysis
      • Enzyme activity assays and chemical measurements
        • Ascorbate peroxidase (APX) activity and ascorbate (Asc) content
        • Glutathione reductase (GR) activity and reduced glutathione (GSH) content
    • Results
      • Short-term high-light treatment of tomato plants
      • Assessment of photosynthetic performance
      • Leaf proteomic profiling
        • General modulation of the proteome in response to light
        • Photosynthesis/carbon fixation
        • Tetrapyrrole pathway
        • Flavonoids
        • Ascorbate and glutathione metabolism
        • Protein co-expression analysis
    • Discussion
      • Photosynthetic performance and changes in related proteins
      • Pigments, antioxidants and photo-protective systems
      • Conclusions
    • Author contributions
    • Declaration of Competing Interests
    • Acknowledgements
    • Supplementary data
    • References

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