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标题:Probucol ameliorates renal injury in diabetic nephropathy by inhibiting the expression of the redox enzyme p66Shc
时间:2020-11-19 22:26:00
DOI:10.1016/j.redox.2017.07.002
PMID:28728079
大小:3299 kb
页数:17 PAGES
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目录:
  • Probucol ameliorates renal injury in diabetic nephropathy by inhibiting the expression of the redox enzyme p66Shc
    • Introduction
    • Results
      • Renal p66Shc, Sirt1 and Ac-H3 expression in DN patients
      • Effects of probucol on biochemical parameters, p66Shc mRNA expression and glomerular injury in diabetic mice
      • Effects of probucol on tubulo-interstitial damage and renal oxidative injury in diabetic mice
      • Probucol treatment regulates renal phospho-AMPK, SIRT1, Ac-H3 and p66Shc expression in diabetic mice
      • Probucol regulates high-glucose-induced p-AMPK, Sirt1, Ac-H3 and P66Shc expression in HK-2 cells
      • Probucol inhibits p66Shc expression through the AMPK/Sirt1 pathway
      • Probucol negatively regulates p66Shc expression via epigenetic chromatin modification
    • Discussion
    • Innovation
    • Materials and methods
      • Cell lines and reagents
      • Kidney morphological analysis
      • Animal experimental design
      • Morphological studies
      • Serum biochemistry
      • Urinary and blood chemistry
      • Kidney AMPK activity
      • SIRT1 deacetylase activity assay
      • Renal tissue immunohistochemistry (IHC) and apoptosis assay
      • Real-time polymerase chain reaction
      • Western blotting procedure
      • Cell culture and treatment
      • Cells immunofluorescence
      • Cellular AMPK measurement
      • Intracellular ROS and malondialdehyde (MDA) measurements
      • Chromatin immunoprecipitation analysis
      • Statistical analysis
    • Author disclosure statement
    • Acknowledgments
    • References

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