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标题:Deproteinization and structural characterization of bioactive exopolysaccharides fromGanoderma sinensemycelium
时间:2020-10-27 10:43:32
DOI:10.1080/01496395.2015.1086375
大小:1434 kb
页数:13 PAGES
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目录:
  • Abstract
  • Introduction
  • Experimental
    • Materials
    • Isolation of crude exopolysaccharides
    • Deproteinization of CEP
      • Deproteinization by the Sevag method
      • Deproteinization by salting out with ammonium sulfate
      • Deproteinization by TCA precipitation
    • Isolation of polysaccharides through anion-exchange chromatography
    • High-performance size-exclusion chromatography (HPSEC)
    • Sugar composition and linkage analysis
    • Amino acid analysis
    • NMR spectroscopy analysis
    • SDS-PAGE
    • Cell lines and cell viability assay
    • Production of cytokines
    • Statistical analysis
  • Results and discussion
    • Deproteinization by the Sevag method
    • Deproteinization by AS salting out
    • Deproteinization by TCA precipitation
    • Isolation and structural characterization of CEP fractions
    • In vitro antitumor activity of CEP fractions
    • Effect of treatment of CEP fractions on cytokines secretion
  • Conclusions
  • Funding
  • References

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