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标题:Controlled release of liposome-encapsulated temozolomide for brain tumour treatment by convection-enhanced delivery
时间:2020-10-26 10:43:51
DOI:10.1080/1061186X.2017.1379526
大小:1077 kb
页数:30 PAGES
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目录:
  • Abstract
  • Introduction
  • Glioblastoma multiforme (GBM) is the most common malignant brain tumor, accounting for approximately 2% of all primary cancers in adults in the United States [1]. Patients typically have a poor prognosis despite current standard therapy with an overal...
  • Materials and Methods
  • Results
  • Characterization of liposomal temozolomide (LipoTMZ)
  • To formulate liposomes containing temozolomide (LipoTMZ) for better drug delivery in a physiological environment, TMZ was loaded into liposomes using the film hydration technique [27, 28]. After encapsulating the TMZ with liposomes and removing the un...
  • Ex vivo release of LipoTMZ and cell viability
  • To study the effect of temperature on LipoTMZ release ex vivo, we examined the TMZ release profile of LipoTMZ at 4(C, 25(C, and 37(C. The release of TMZ over 72 h was very slow at 4(C and 25(C, and increased quite obviously once the temperature chang...
  • To test the cellular uptake and subcellular distribution of the drug and its release, the liposomes were labeled with a fluorescent dye, DiI, and incubated with U87MG cells for 24, 48, and 72 h. The images showed that the LipoDiI bound the cell surfa...
  • Tumor-bearing mice treated with CED-LipoTMZ had significantly reduced tumor growth and improved survival times compared to the control groups. Since TMZ is an alkylating agent that targets proliferative cells, and tumor cells do not undergo cell divis...
  • Despite the advantages of CED-LipoTMZ described here, other practical obstacles need to be solved before the method can be applied for clinical management of brain tumors. An appropriate imaging system is required to monitor and confirm drug distribut...

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