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标题:Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway.
时间:2020-10-18 16:26:07
大小:2019 kb
页数:11 PAGES
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目录:
  • Decreased NHE3 activity and trafficking in TGEV-infected IPEC-J2 cells via the SGLT1-mediated P38 MAPK/AKt2 pathway
    • Introduction
    • Materials and methods
      • Cells and viruses
      • Lentivirus-mediated RNA interference
      • Inhibitor
      • Western blot
      • RT-qPCR
      • IFA analyses
      • Determination of the intracellular and extracellular sodium ion concentration
      • Measurement of the Na+/H+ exchange activity
      • Measurement of surface NHE3 expression
      • Statistics
    • Results
      • TGEV infection is related to the expression of surface NHE3 and the activity of Na+/H+ exchange
      • Regulation of SGLT1 on NHE3 translocation in mock-infected cells
      • Inhibition of SGLT1 promotes the translocation of NHE3 in TGEV-infected cells
      • Inhibition of SGLT1 promotes Na+ absorption in TGEV-infected cells
    • Discussion
    • CRediT authorship contribution statement
    • mk:H1_20
    • Acknowledgments
    • References

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