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标题:Poxvirus K7 Protein Adopts a Bcl-2 Fold: Biochemical Mapping of Its Interactions with Human DEAD Box RNA Helicase DDX3
时间:2020-10-18 13:52:27
DOI:10.1016/j.jmb.2008.09.048
大小:838 kb
页数:12 PAGES
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目录:
  • Poxvirus K7 Protein Adopts a Bcl-2 Fold: Biochemical Mapping of Its Interactions with Human DEA.....
    • Introduction
    • Results
      • Solution structure of K7R
      • K7 is a monomer with a closed BH3 groove
      • Backbone dynamics of K7
      • Mapping of K7 interactions with human RNA helicase DDX3
    • Discussion
    • Materials and Methods
      • Protein expression and purification
      • Gel-filtration chromatography
      • In vitro pull-down experiments
      • NMR spectroscopy and structure determination
      • Isothermal titration calorimetry
      • Protein Data Bank accession numbers
    • Acknowledgements
    • Supplementary Data
    • References

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