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标题:TXNIP regulates mitophagy in retinal Müller cells under high-glucose conditions: implications for diabetic retinopathy
时间:2020-10-14 22:26:08
DOI:10.1038/cddis.2017.190
PMID:28492550
大小:7060 kb
页数:13 PAGES
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目录:
  • title_link
    • Figure 1 High glucose induces TXNIP expression and mitochondrial dysregulation in rMC1 in culture.
    • Results
      • HG induces TXNIP expression and mitochondrial localization and dysfunction in rat retinal Müller cells
    • Figure 2 High glucose mediates macroautophagy/mitophagy induction in rMC1.
      • HG induces mitophagy in rMC1
    • Figure 3 High glucose induces damaged MT flux to lysosomes in rMC1.
      • TXNIP knockout prevents mitophagy in rMC1 under HG conditions
    • Figure 4 TXNIP knockout prevents mitophagy in rMC1 under high-glucose conditions.
    • Figure 5 Mitophagy is reduced in T(1+3) rMC1’cells under high-glucose conditions.
    • Figure 6 High glucose induces ubiquitination of mitochondrial membrane protein in rMC1, but not in T(1+3) cells.
      • A potential mechanism for TXNIP-mediated mitophagy in rMC1
      • TXNIP expression is strongly induced in the diabetic rat retina and participates in LC3BII puncta formation
    • Discussion
    • Figure 7 High glucose increases localization of fission protein Drp1 and E3 ubiquitin ligase Parkin in mitochondria in rMC1’cells, but not in TXNIP knockout T(1+3) cells.
    • Figure 8 Potential mechanisms for TXNIP-mediated mitophagy in retinal Müller cells under high-glucose environment.
    • Materials and Methods
      • Materials
      • Methods
        • Cell culture
        • Mitochondrial isolation
        • MitoSOX assay
        • Mitochondrial membrane potential measurement by JC1
        • CRISPR/Cas9 and TXNIP gRNA design and cleavage efficiency assay
        • Western blotting and QPCR
        • Immunofluorescence
      • Diabetes induction in rats
      • Statistical analysis
      • NIH/NEI: EY023992 to LPS; EY014370, EY017313 and EY022230 to RAK; Core. P30EY004068 to the Department of Anatomy and Cell Biology; and unrestricted Research to Prevent Blindness grant to the Department of Ophthalmology. Language editing help from American
    • ACKNOWLEDGEMENTS
      • Supplementary Information accompanies this paper on Cell Death and Disease website (http://www.nature.com/cddis)Edited by GM’FimiaSingh LP, Perrone L. Thioredoxin interacting protein (TXNIP) and pathogenesis of diabetic retinopathy. J Clin Exp Ophthalmol

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