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标题:QKI deficiency maintains glioma stem cell stemness by activating the SHH/GLI1 signaling pathway
时间:2020-10-10 22:24:48
DOI:10.1007/s13402-019-00463-x
PMID:31292920
大小:10456 kb
页数:14 PAGES
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目录:
  • QKI deficiency maintains glioma stem cell stemness by activating the SHH/GLI1 signaling pathway
    • Abstract
    • Abstract
    • Abstract
    • Abstract
    • Abstract
    • Introduction
    • Materials and methods
      • Glioma datasets and tissue specimens
      • Cell lines and culture conditions
      • Plasmid construction and lentivirus infection
      • Cell proliferation and invasion assays
      • Neurosphere formation and limiting dilution assays
      • Western blot analysis
      • RT-PCR and agarose gel electrophoresis
      • RNA-binding protein immunoprecipitation (RIP)
      • Immunohistochemistry
      • Nude mouse intracranial model
      • Statistical analysis
    • Results
      • QKI expression is decreased in GSCs and is associated with a better survival of GBM patients
      • QKI enhances the proliferation, but impairs the stemness and invasive ability of GSCs
      • QKI silencing inhibits the proliferation, but enhances the stemness and invasive ability of GSCs
      • QKI expression promotes phosphorylation of the AKT/ERK pathway and reduces GLI1 expression in GSCs
      • QKI silencing enhances stemness by activating the Hedgehog signaling pathway in GSCs
      • QKI inhibits glioma stemness, reduces tumor growth and prolongs mouse survival
    • Discussion
    • References

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