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标题:Enhancement of Liposomal Plasmid DNA and siRNA Delivery by Itraconazole through Intracellular Cholesterol Accumulation
时间:2020-09-17 09:34:32
DOI:10.1007/s11095-020-02846-4
大小:1909 kb
页数:10 PAGES
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目录:
  • Enhancement of Liposomal Plasmid DNA and siRNA Delivery by Itraconazole through Intracellular Cholesterol Accumulation
    • Abstract
    • Abstract
    • Abstract
    • Abstract
    • Abstract
    • Introduction
    • Materials and Methods
      • Cell Line and Materials
      • Plasmid DNA Transfection
      • Luciferase Assay
      • B Cell Lymphoma 2 (Bcl2) siRNA Transfection
      • Cell Viability Assay
      • RNA Extraction and Real Time qPCR
      • Western Blot
      • Filipin Staining
      • Observation of Confocal Microscope
      • Statistical Analysis
    • Results
      • Cholesterol and Itraconazole Increases Liposomal Plasmid DNA Transfection
      • Itraconazole Increases Bcl2 siRNA Transfection
      • Itraconazole Enhances Gene Delivery Via Intracellular Cholesterol Accumulation
      • Co-Treatment with Itraconazole and Imipramine Additively Enhances pDNA and siRNA Delivery
    • Discussion
    • Conclusion
    • References

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