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标题:Evaluation of mixed-mode chromatographic resins for separating IgG from serum albumin containing feedstock
时间:2020-09-17 00:25:55
DOI:10.1016/j.jchromb.2013.07.029
大小:1993 kb
页数:10 PAGES
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目录:
  • Evaluation of mixed-mode chromatographic resins for separating IgG from serum albumin containing feedstock
    • 1 Introduction
    • 2 Experimental
      • 2.1 Materials
      • 2.2 Chromatographic separation with the pH gradient elution
      • 2.3 Chromatographic separation with sequential pH step elution
      • 2.4 SEC-HPLC analysis
      • 2.5 Analysis by non-reducing SDS-PAGE
      • 2.6 Determination of zeta potential
    • 3 Results and discussion
      • 3.1 Properties of resins and proteins
      • 3.2 Separation performance of four resins
        • 3.2.1 Bestarose Diamond MMA
        • 3.2.2 Bestarose Diamond MMC
        • 3.2.3 MEP HyperCel
        • 3.2.4 PPA HyperCel
        • 3.2.5 Comparison analysis
      • 3.3 Effects of mobile phase modifiers with Bestarose Diamond MMA
      • 3.4 Optimizing the separation performance with pH stepwise elution
      • 3.5 Scale-up effects of sample loading
    • 4 Conclusions
    • Acknowledgments
    • Appendix A Supplementary data
    • Appendix A Supplementary data

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