在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:Acetylation Blocks cGAS Activity and Inhibits Self-DNA-Induced Autoimmunity
时间:2020-09-16 21:31:00
DOI:10.1016/j.cell.2019.01.016
大小:4956 kb
页数:30 PAGES
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

目录:
  • Acetylation Blocks cGAS Activity and Inhibits Self-DNA-Induced Autoimmunity
    • Introduction
    • Results
      • Acetylation-Mimicking Mutation of cGAS Blocks cGAS Activation
      • Acetylation Suppresses cGAS Activity
      • Deacetylation of cGAS upon DNA Treatment Is Involved in cGAS Activation
      • Enforced Acetylation of cGAS by Aspirin
      • Aspirin Inhibits cGAS-Mediated IFN Production
      • Aspirin Suppresses DNA-Mediated Autoimmunity in Mice
      • Aspirin Suppresses DNA-Mediated Autoimmunity in Human Patient Cells
      • Aspirin Suppresses cGAS-Mediated Type I Interferonopathies
    • Discussion
    • Supplemental Information
    • Acknowledgments
    • Author Contributions
    • Declaration of Interests
    • References
    • STAR★Methods
      • Key Resources Table
      • Contact for Reagent and Resource Sharing
      • Experimental Models and Subject Details
        • Mice
        • Cells
        • Viruses
      • Methods Details
        • Quantitative Polymerase Chain Reaction (qPCR)
        • ELISA
        • Isolation of PBMCs from AGS patient
        • Mass Spectrometry
        • Immunoblotting and Immunoprecipitation
        • Purification of site-specific acetylated cGAS recombinant proteins
        • In vitro cGAMP synthesis assay
        • cGAMP extraction
        • Quantification of cGAMP using LC–MS/MRM
        • RNA Interference
        • IC50 Determination
        • DNA Binding Assay
        • Immunofluorescence
        • cGAS dimerization
      • Quantification and Statistical Analysis
      • Data and Software Availability

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系