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标题:Multiway real-time PCR gene expression profiling in yeast Saccharomyces cerevisiae reveals altered transcriptional response of ADH-genes to glucose stimuli
时间:2020-09-16 16:32:37
DOI:10.1186/1471-2164-9-170
大小:1003 kb
页数:16 PAGES
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目录:
  • Abstract
    • Background
    • Results
    • Conclusion
  • Background
  • Results and Discussion
    • Experimental setup and gene selection
    • Validation of reference genes
    • Data pre-processing
    • Classification of the genes' expression profiles with principal component analysis
    • Confirmation with hierarchical clustering and Kohonen self-organizing maps
  • Conclusion
  • Methods
    • Strains and growth conditions
    • RNA extraction, cDNA synthesis and quantitative real- time PCR
  • Authors' contributions
  • Additional material
  • Acknowledgements
  • References

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