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标题:A comparative analysis of protein virulence factors released via extracellular vesicles in two Candida albicans strains cultivated in a nutrient-limited medium
时间:2020-09-13 17:08:43
DOI:10.1016/j.micpath.2019.103666
PMID:31412284
大小:999 kb
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目录:
  • A comparative analysis of protein virulence factors released via extracellular vesicles in two Candida albicans strains cultivated in a nutrient-limited medium
    • Introduction
    • Materials and methods
      • Fungal strains, growth conditions, growth curve mapping
      • Extracellular vesicles isolation and purification
      • LC-MS/MS, label-free quantification, data analysis
      • Microscopic visualization of extracellular vesicles by transmission electron microscopy
      • Bioinformatic analysis
      • Statistical analysis
    • Results and discussion
      • Determination of the optimal cultivation period for EVs harvesting with limited risk of contamination
      • Confirmation of the presence of EVs by electron microscopy
      • New insights into the presence of virulence factors in C. albicans EVs revealed by a proteomic analysis
      • Members from families of the key virulence-associated protein are trafficking via C. albicans EVs
      • C. albicans virulence factors are released prominently via EVs in extracellular milieu
      • A statistical analysis revealed a not-trivial difference in the quantity of proteins released from two C. albicans strains via EV trafficking
    • Conclusion
    • Conflicts of interest
    • Acknowledgement/Funding
    • Supplementary data
    • References

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