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标题:A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor and associates with ABA signaling
时间:2020-08-08 10:57:33
DOI:10.1007/s11103-006-9079-x
大小:643 kb
页数:20 PAGES
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目录:
  • A rice dehydration-inducible SNF1-related protein kinase 2 phosphorylates an abscisic acid responsive element-binding factor and associates with ABA signaling
  • Abstract
  • Introduction
  • Materials and methods
  • Construction and screening of cDNA library
  • Northern blot analysis
  • RT-PCR analysis of gene expression
  • Cloning of OREB1 and lip19
  • Site-direct mutagenesis
  • Construction of plasmids
  • Expression and purification of GST-fusion proteins in E. coli
  • In vitro Protein kinase assays
  • Peptide mass fingerprinting by MALDI-TOF MS/MS
  • Yeast two hybrid assays
  • Fluorescence microscopy
  • Tobacco transformation and growth
  • Results
  • OSRK1 encodes a serine/threonine protein kinase of SnRK2 family subgroup1
  • OSRK1 is not a rapid responsive gene by hyperosmotic stress signal
  • Bacterial recombinant GST-OSRK1 protein showed kinase activity with strong substrate preference for rice bZIP proteins and uncommon cofactor requirement
  • Fig1
  • Fig2
  • Regulation of intrinsic biochemical activity of OSRK1 in vitro
  • Fig3
  • OSRK1 phosphorylates OREB1, a rice homolog of ABF/AREB/ABI5 subfamily of bZIP transcription factor at multiple functional domains
  • Fig4
  • Identification of phosphorylation sites of GST-D1 protein by OSRK1
  • Fig5
  • OSRK1 interacts with OREB1 in a yeast two-hybrid system and co-localized to nuclei
  • Fig6
  • Fig7
  • Transgenic tobacco expressing OSRK1 shows mild ABA insensitive phenotype
  • Fig8
  • Fig9
  • Discussion
  • Fig10
  • Fig11
  • Fig12
  • Acknowledgements
  • References

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