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标题:Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage
时间:2020-08-02 08:13:54
DOI:10.1016/j.cell.2020.03.052
大小:15277 kb
页数:42 PAGES
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目录:
  • Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage
    • Introduction
    • Results
      • Cells Show a Rapid Chromatin and a Slow Supracellular Mechanoresponse with Distinct Amplitude Dependencies
      • Stretch-Triggered Heterochromatin Changes Occur Mainly at Non-coding Regions with No Substantial Correlation with Transcrip ...
      • Stretch Induces Changes in NE Tension and Nuclear Mechanical Properties
      • H3K9me3 Heterochromatin Regulates Nuclear Mechanics and Chromatin Mobility to Prevent DNA Damage
      • Chromatin Mechanoresponse Is Mediated by Nuclear-Deformation-Triggered Intracellular Calcium
      • Minimum Nuclear Stiffness Is Required for Ca2+ Release and Subsequent Heterochromatin Response
      • Supracellular Monolayer Alignment Prevents Stress Transmission to the Nucleus and Chromatin
    • Discussion
    • Supplemental Information
    • Acknowledgments
    • Author Contributions
    • Declaration of Interests
    • References
    • STAR★Methods
      • Key Resources Table
      • Lead Contact and Materials Availability
      • Experimental Model and Subject Details
        • Primary cells and cell lines
        • Mice
      • Method Details
        • Cell culture
        • Mechanical stretching
        • Chemical treatments
        • Transfections, plasmids and RNAi
        • Immunofluorescence and confocal microscopy
        • Image analyses
        • Mass spectrometry
        • RNA sequencing and analysis
        • Chromatin immunoprecipitation
        • ChIP sequencing and analysis
        • Western blotting
        • Atomic force microscopy (AFM)
        • Chromatin mobility/rheology
        • Electron microscopy
        • Electron tomography
        • Membrane tension measurements
        • Super resolution microscopy
        • Calcium and nuclear deformation imaging
        • qPCR
        • Nuclear compression
        • Mice, ex vivo stretch and immunofluorescence of tissue
      • Quantification and Statistical Analysis
      • Data and Code Availability

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