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标题:Tetramethylpyrazine Attenuated Sevoflurane-Induced Neurotoxicity by Enhancing Autophagy through GPR50/CREB Pathway in SH-SY5Y Cells
时间:2020-08-02 02:53:29
DOI:10.1142/s0192415x20500457
大小:1429 kb
页数:23 PAGES
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目录:
  • Tetramethylpyrazine Attenuated Sevoflurane-Induced Neurotoxicity by Enhancing Autophagy through GPR50/CREB Pathway in SH-SY5Y Cells
    • Introduction
    • Materials and Methods
      • Cell Culture
      • Drug Treatment
      • Western Blot Analysis for GPR50, CREB, pCREB, Atg5, Beclin-1, LC3B, Bax, Bcl-2, BACE1, and Aβ
      • Quantitative Real-Time Polymerase Chain Reaction (QRT-PCR) Assay for BECN1, Atg5, BACE1, and β-APP
      • Immunofluorescence Analysis for BACE1
      • mRFP-GFP-LC3 Expression Plasmid Transient Transfection
      • Small Interference RNA (siRNA) Atg5 and siRNA GPR50 Transient Transfection
      • Apoptotic Cell Analysis
    • Statistical Analysis
    • Results
      • Sevoflurane Activated Autophagy in Neonatal Mice and SH-SY5Y Cells
      • Sevoflurane Induced Apoptosisin SH-SY5Y Cells
      • Upregulation of Autophagy Attenuated Sevoflurane-Induced Apoptosis in SH-SY5Y Cells
      • Sevoflurane Increased Expression of BACE1 and Aβ in SH-SY5Y Cells
      • TMP Potentiated Sevoflurane-Induced Autophagy
      • TMP Alleviated Sevoflurane-Induced Apoptosis
      • TMP Attenuated Sevoflurane-Induced Apoptosis by Enhancing Autophagy
      • TMP Decreased Sevoflurane-Induced Increased Expression of BACE1 and Aβ
      • Silencing of Atg5 Increased Expression of BACE1and Aβ in SH-SY5Y Cells After Exposure to Sevoflurane and TMP
      • Suppression of GPR50 Decreased Expression of pCREB, Atg5, and LC3-II in SH-SY5Y Cells after Exposure to Sevoflurane and TMP
    • Discussion
    • Acknowledgments
    • References

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