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标题:Suppression of Exosomal PD-L1 Induces Systemic Anti-tumor Immunity and Memory
时间:2020-07-28 16:40:41
DOI:10.1016/j.cell.2019.02.016
PMID:30951669
大小:5421 kb
页数:29 PAGES
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目录:
  • Suppression of Exosomal PD-L1 Induces Systemic Anti-tumor Immunity and Memory
    • Introduction
    • Results
      • Differential Secretion of PD-L1 between Cancer Cell Lines
      • PD-L1 Is Specifically Secreted within Exosomes
      • Exosomal PD-L1 Suppresses T Cell Activation In Vitro
      • Exosomal PD-L1 Promotes Tumor Progression
      • Exosomal PD-L1 Suppresses T Cell Activity in the Draining Lymph Node
      • Exosomal PD-L1 Promotes Growth across Different Tumor Types
      • Exosomal PD-L1-Deficient Tumor Cells Can Suppress WT Tumor Growth at a Distance Site
      • Exogenously Introduced Exosomal PD-L1 Can Rescue Immune Suppression and Tumor Growth
    • Discussion
    • Supplemental Information
    • Acknowledgments
    • Author Contributions
    • Declaration of Interests
    • References
    • STAR★Methods
      • Key Resources Table
      • Contact for Reagent and Resource Sharing
      • Experimental Model and Subject Details
        • Human tumor cell lines
        • Mouse tumor cell lines
        • Mice
      • Method Details
        • Cell lines treatments
        • Exosome/Secreted Vesicle isolation
        • CRISPR–Cas9-mediated gene disruption
        • Electron Microscopy
        • Nanoparticle Tracking Analysis
        • Antibodies
          • Immunoblotting
          • Flow Cytometry
          • Immunofluorescence
        • Western Blot Analysis
        • Raji - Jurkat T cell stimulation assay
        • Immunofluorescence
        • Polysome Fractionation and RNA isolation
        • Reverse transcriptase-quantitative PCR
          • Pd-l1 qPCR forward primer
          • Rpl7 qPCR forward primer
        • Cell Fractionation
        • Biotin-Streptavidin Assay
        • Tumor cells injections
        • Mice Treatments
        • Immune-Profiling
        • Haematoxylin and eosin staining
      • Quantification and Statistical Analysis
      • Data Availability

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