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标题:Effective MSTN Gene Knockout by AdV-Delivered CRISPR/Cas9 in Postnatal Chick Leg Muscle
时间:2020-07-26 19:47:01
DOI:10.3390/ijms21072584
PMID:32276422
大小:1618 kb
页数:13 PAGES
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目录:
  • Introduction
  • Results
    • MSTN Knockout (KO) in the Muscles of Chicks
    • Differentially Expressed Genes (DEGs) in KO Muscles
    • Gene Ontology (GO) and Pathway Analyses of DEGs
  • Discussion
  • Materials and Methods
    • Plasmid Construction
    • Feasibility Assessment of the AdV-CRISPR System in DF-1 Cells
    • Fertilized Eggs, Incubation, and Chick Breeding
    • Intramuscular Injection of the AdV-CRISPR System
    • Detection of Mutations in the MSTN Gene
    • Transcriptome Sequencing and Differentially Expressed Gene (DEG) Analysis
    • Gene Ontology (GO) and Pathway Analysis of Differentially Expressed Genes (DEGs)
    • Ethics Statement
  • References

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