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标题:Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells
时间:2020-07-26 17:23:21
DOI:10.1016/j.ygeno.2019.08.020
PMID:31465786
大小:1984 kb
页数:11 PAGES
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目录:
  • Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells
    • Introduction
    • Methods
      • Cell lines and reagents
      • Knockdown of miR-214 using CRISPR/Cas 9 technique and checking gene expression by RNA extraction followed by quantitative RT-PCR
      • Over-expression of miR-214 by pCDNA-HisA plasmid transfection and checking gene expression by RNA extraction followed by quantitative RT-PCR
      • Apoptosis assay after knockdown and over-expression of miR-214
      • Genomic DNA extraction and confirming miR-214 gene knockdown and overexpression using real-time PCR
      • RNA extraction and library construction
      • RNA sequencing and data analysis
      • Biological pathways and gene ontology enrichment analysis
      • RNA extraction and quantitative RT-PCR
    • Results
      • Confirmation of miR-214 knockout and overexpression in cervical cancer cell lines
      • Differential expression of mRNAs in cells having miR-214 knockout and overexpression respectively
      • Gene ontology and KEGG pathway analysis of differentially expressed mRNAs
      • Gene networks regulated by miR-214
      • Validation of NGS data by quantitative real-time PCR
    • Discussion
    • Conclusion
    • Statement of ethics
    • Disclosure statement
    • Funding sources
    • Authors' contributions
    • Acknowledgments
    • References

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