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标题:Generation and evaluation of a vaccine candidate of attenuated and heat-resistant genotype VIII Newcastle disease virus
时间:2020-07-07 17:50:46
DOI:10.1016/j.psj.2020.01.034
大小:919 kb
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目录:
  • Generation and evaluation of a vaccine candidate of attenuated and heat-resistant genotype VIII Newcastle disease virus
    • Introduction
    • Materials and methods
      • Virus, cells, and plasmid
      • Construction of NDV Reverse Genetic and Modification of the F Protein Cleavage Site
      • Characterization of the Rescued Virus
      • Immunization and Challenge Studies in SPF Chickens
      • Assessment of Antibody Levels
      • Virus Shedding Analysis
      • Ethics Statements
      • Statistical Analysis
    • Results
      • Comparison of the Amino Acid Sequences of the F and HN Proteins
      • Generation and Identification of the NDV/rHR09 Viruses
      • Biological Characterization of the NDV Strains
      • Thermostability and Growth Kinetics
      • Antibody Response and Survival Rate
      • Reduction in Virulent NDV Shedding
    • Discussion
    • Acknowledgments
    • References

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