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标题:TRAIL enhances quinacrine-mediated apoptosis in breast cancer cells through induction of autophagy via modulation of p21 and DR5 interactions
时间:2020-07-01 11:55:37
DOI:10.1007/s13402-017-0347-3
大小:10545 kb
页数:16 PAGES
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目录:
  • TRAIL...
    • Abstract
    • Abstract
    • Abstract
    • Abstract
    • Abstract
    • Introduction
    • Materials and methods
      • Cell culture and reagents
      • Cell treatment
      • Western blotting
      • Acridine orange (AO) staining
      • Death inducing signaling complex (DISC) isolation
      • Cell viability assay
      • Apoptosis measurement using Annexin V-FITC/PI dual staining
      • Intracellular ROS and RNS measurements
      • Co-localization of p21 and DR5
      • p21 and DR5 interaction analysis
      • Acridine orange Hoechst dual staining
      • DR5 gene silencing
      • p21 gene silencing
      • Development of a mouse xenograft model
      • H&E staining
      • Immunohistochemistry (IHC)
      • Double immunostaining of mouse tissue sections
      • Statistical analyses
    • Results
      • TRAIL enhances QC-mediated autophagy in MCF-10A-Tr cells
      • QC along with TRAIL induces apoptosis in MCF-10A-Tr cells via autophagy
      • TRAIL and QC induce autophagy and apoptosis in MCF-10A-Tr cells through p21 and DR5 interaction
      • Silencing of DR5 leads to reduced autophagy and apoptosis in MCF-10A-Tr cells
      • Silencing of both p21 and DR5 leads to autophagy and apoptosis reduction in MCF-10A-Tr cells
      • TRAIL + QC treatment causes apoptosis and autophagy in MCF-7 cells via up regulation of p21 and DR5
      • TRAIL + QC exposure upregulates p21 and DR5 expression in a mouse xenograft model
    • Discussion
    • References

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