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标题:Targeting elongation factor-2 kinase (eEF-2K) induces apoptosis in human pancreatic cancer cells
时间:2020-07-01 11:47:50
DOI:10.1007/s10495-013-0927-2
大小:1249 kb
页数:19 PAGES
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目录:
  • Targeting elongation factor-2 kinase (eEF-2K) induces apoptosis in human pancreatic cancer cells
    • Abstract
    • Introduction
    • Materials and methods
      • Cell lines, culture conditions and reagents
      • Transfections with siRNA
      • Packaging of pCDH constructs in pseudoviral particles and production of lentivirus
      • Transduction of PANC-1 cells with eEF-2K lentiviral expression vectors
      • Cell viability and proliferation/growth assays
      • Western blot analysis
      • RNA isolation and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis
      • Analysis of cell death
      • Analysis of mitochondrial activity and live cells
      • Reverse phase protein arrays (RPPA)
      • Statistical analysis
    • Results
      • eEF-2K is overexpressed in PaCa cells
      • Down-regulation of eEF-2K expression may underlie ROT-mediated inhibition of PaCa cell proliferation
      • ROT down-regulates eEF-2K protein and gene expression
      • ROT treatment stimulates ubiquitin-mediated proteasomal degradation of eEF-2K in PaCa cells
      • Knockdown of eEF-2K leads to down-regulation of TG2 expression independently of PKC- delta
      • ROT stimulates intrinsic apoptotic-death of PaCa cells
      • Down-regulation of eEF-2K contributes to ROT-induced apoptosis independently of PKC- delta in PaCa cells
      • eEF-2K inhibition contributes to ROT-induced disruption of mitochondrial membrane potential ( Delta Psi m)
      • eEF-2K down-regulation contributes to ROT-induced stimulation of extrinsic apoptotic pathway
      • Inhibition of eEF-2K/TG2 axis leads to induction of apoptosis inducing factor (AIF) in PaCa cells
      • eEF-2K over-expression suppresses ROT-mediated apoptosis in PaCa cells
    • Discussion
    • Acknowledgments
    • References

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