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标题:Deletion of cas3 gene in Streptococcus mutans affects biofilm formation and increases fluoride sensitivity
时间:2020-06-30 13:52:49
DOI:10.1016/j.archoralbio.2019.01.016
大小:2161 kb
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目录:
  • Deletion of cas3 gene in Streptococcus mutans affects biofilm formation and increases fluoride sensitivity
    • Introduction
    • Materials and methods
      • Bacterial strains and growth conditions
      • Construction of cas3 gene deletion mutant
      • Complementation of the cas3 mutation in trans
      • Growth curve measurement
      • Crystal violet staining
      • Quantitative determination of water-insoluble EPS
      • Biofilm analysis and structural imaging
      • Dual-species interaction within the biofilm
      • Fluoride tolerance assays
      • Quantitative real-time PCR (qRT-PCR)
      • Statistical analysis
    • Results
      • Deletion of cas3 in S. mutans leads to decreased biofilm biomass and EPS production
      • S. mutans Δcas3 strain forms less and thinner biofilm
      • S. mutans Δcas3 exhibits increased sensitivity to fluoride and compromised competitiveness against S. sanguinis under fluoride treatment
      • Expression of biofilm formation related gene in S. mutans lacking cas3
    • Discussion
    • Conclusions
    • Competing interests
    • Author contributions
    • Conflict of interests
    • Sources of funding
    • Ethical approval
    • Acknowledgements
    • Supplementary data
    • References

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