| 标题： | Deletion of cas3 gene in Streptococcus mutans affects biofilm formation and increases fluoride sensitivity |
| 时间： | 2020-06-30 13:52:49 |
| DOI： | 10.1016/j.archoralbio.2019.01.016 |
| 大小： | 2161 kb |
| 下载： | 点击下载 |
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目录：
- Deletion of cas3 gene in Streptococcus mutans affects biofilm formation and increases fluoride sensitivity
- Introduction
- Materials and methods
- Bacterial strains and growth conditions
- Construction of cas3 gene deletion mutant
- Complementation of the cas3 mutation in trans
- Growth curve measurement
- Crystal violet staining
- Quantitative determination of water-insoluble EPS
- Biofilm analysis and structural imaging
- Dual-species interaction within the biofilm
- Fluoride tolerance assays
- Quantitative real-time PCR (qRT-PCR)
- Statistical analysis
- Results
- Deletion of cas3 in S. mutans leads to decreased biofilm biomass and EPS production
- S. mutans Δcas3 strain forms less and thinner biofilm
- S. mutans Δcas3 exhibits increased sensitivity to fluoride and compromised competitiveness against S. sanguinis under fluoride treatment
- Expression of biofilm formation related gene in S. mutans lacking cas3
- Discussion
- Conclusions
- Competing interests
- Author contributions
- Conflict of interests
- Sources of funding
- Ethical approval
- Acknowledgements
- Supplementary data
- References
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