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标题:Unsupervised High-Dimensional Analysis Aligns Dendritic Cells across Tissues and Species
时间:2020-06-30 13:26:16
DOI:10.1016/j.immuni.2016.08.015
大小:8635 kb
页数:18 PAGES
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目录:
  • Unsupervised High-Dimensional Analysis Aligns Dendritic Cells across Tissues and Species
    • Introduction
    • Results
      • A Unique Gating Strategy Allows the Identification of cDC1s and cDC2s across Mouse Tissues
      • Inclusion of CD24 Permits the Separation of Langerhans Cells from Dermal cDC2s
      • Kidney CD64+ cDC2 Can Be Identified by Their CD26hiCD11chiIRF8loIRF4hi Profile
      • Unsupervised Analysis Aligns cDC1 and cDC2 across Mouse Tissues
      • Unsupervised Analysis of Mutant Mouse Strains Using FlowSOM and tSNE Outperforms Manual Analysis
      • A Conserved Gating Strategy Aligns cDC Subsets in Mouse, Human, and Macaque Tissues
      • tSNE Automatically Identifies cDCs across Tissues and Species
      • One-SENSE Analysis of CyTOF Data Reveals Heterogeneity of DC Subsets across Mouse and Human Tissues
      • One-SENSE Analysis of CyTOF Data Automatically Delineates DC Activation during Inflammation
    • Discussion
    • Experimental Procedures
      • Mice
      • Intranasal LPS
      • Human Tissues
      • Flow Cytometry Stainings
      • Mass Cytometry Stainings, Barcoding, Acquisition, and Data Pre-processing
      • FlowSOM and tSNE-Based Unsupervised Analysis
    • Supplemental Information
    • Author Contributions
    • Acknowledgments
    • References

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