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标题:Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids
时间:2020-05-23 09:29:20
DOI:10.1016/j.ymben.2016.01.006
大小:1391 kb
页数:10 PAGES
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目录:
  • Experimental and computational optimization of an Escherichia coli co-culture for the efficient production of flavonoids
    • Introduction
    • Materials and methods
      • Bacterial Strains, vectors, and media
      • Flavonoid pathways and ePathOptimize library construction
      • Small-scale cultivation protocol
      • Bioreactor fermentation protocol
      • Metabolite analysis
      • Empirical modeling methods
    • Results and discussion
      • Independent optimization of upstream and downstream modules
      • Determination of co-culture compatibility
      • Determination of important optimization parameters
      • Effect of carbon source
      • Induction temperature optimization
      • System modeling for prediction of optimum operating conditions
      • Bioreactor scale-up: proof of principle
    • Conclusions
    • Conflicts of interest
    • Author contributions
    • Acknowledgments
    • Supplementary material
    • References

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