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标题:Improving arterial prosthesis neo-endothelialization: application of a proactive VEGF construct onto PTFE surfaces.
时间:2020-03-26 21:57:30
DOI:10.1016/j.biomaterials.2005.05.051
PMID:16005960
作者:M. Crombez; P. Chevallier; R.C. -Gaudreault
关键词:Surface modification;Endothelial cells;Plasma treatment
摘要:http://linkinghub.elsevier.com/retrieve/pii/S0142961205004631
大小:367 kb
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目录:
  • Improving arterial prosthesis neo-endothelialization: Application of a proactive VEGF construct onto PTFE surfaces
    • Introduction
    • Materials and methods
      • Materials
      • Method
        • Plasma treatment
        • Glutaric anhydride grafting
        • Activation of the surface carboxylic acid moieties with EDC
        • Conjugation of HSA on EDC-grafted PTFE surfaces
        • Loading the HSA-grafted surface with negative charges
        • XPS analyses
        • VEGF seeding and detection
        • Human ECs adhesion and migration
    • Results and discussion
      • Plasma treatment and glutaric anhydride grafting
      • Conjugation and characterization of the negatively charged matrix on the polymer surface
      • VEGF loading and immunodetection
    • Conclusion
    • Acknowledgements
    • References

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