在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:Identification and characterization of a new human type 9 cGMP-specific phosphodiesterase splice variant (PDE9A5)
时间:2020-03-26 21:50:08
DOI:10.1016/S0378-1119(03)00733-9
大小:1800 kb
页数:14 PAGES
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

目录:
  • Identification and characterization of a new human type 9 cGMP-specific phosphodiesterase splice variant (PDE9A5)
    • Introduction
    • Materials and methods
      • Cloning of PDE9A5 cDNA
      • Analysis of PDE9A DNA and protein sequences
      • Expression of recombinant PDE9A5 and PDE9A1 proteins and preparation of subcellular fractions
      • Enzyme assay of recombinant PDE9A5 and PDE9A1
      • Quantification of recombinant PDE9A5 and PDE9A1 in crude cellular extracts
      • Western blot analysis of recombinant PDE9A5 and PDE9A1
      • Immunofluorescence microscopy of recombinant PDE9A5 and PDE9A1
      • Site-directed mutagenesis of PDE9A1
      • Determination of tissue distribution of PDE9A5 and PDE9A1 mRNAs
      • Preparation of subcellular fractions of human blood T cells and western blot analysis of native PDE9A1
    • Results
      • Identification, cloning and analysis of full-length PDE9A5 cDNA
      • Tissue distribution of PDE9A5 and PDE9A1 mRNAs
      • Subcellular localization of recombinant full-length PDE9A5 and PDE9A1 proteins
      • Subcellular localization of native PDE9A1 protein
      • Enzymatic properties of recombinant full-length PDE9A5 and PDE9A1 proteins
    • Discussion
    • Acknowledgements
    • References

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系