在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate.
时间:2020-02-15 06:24:53
DOI:10.1038/s41563-017-0014-0
PMID:29434303
作者:von Erlach, Thomas C.; Bertazzo, Sergio; Wozniak, Michele A.
出版源: 《Nature Materials》
摘要:Cell size and shape affect cellular processes such as cell survival, growth and differentiation1,2,3,4, thus establishing cell geometry as a fundamental regulator of cell physiology. The contributions of the cytoskeleton, specifically actomyosin tension, to these effects have been described, but the exact biophysical mechanisms that translate changes in cell geometry to changes in cell behaviour remain mostly unresolved. Using a variety of innovative materials techniques, we demonstrate that the nanostructure and lipid assembly within the cell plasma membrane are regulated by cell geometry in a ligand-independent manner. These biophysical changes trigger signalling events involving the serine/threonine kinase Akt/protein kinase B (PKB) that direct cell-geometry-dependent mesenchymal stem cell differentiation. Our study defines a central regulatory role by plasma membrane ordered lipid raft microdomains in modulating stem cell differentiation with potential translational applications.
大小:2552 kb
页数:11 PAGES
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

目录:
  • Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate
    • Methods
    • Acknowledgements
    • Fig. 1 Cell geometry induces changes in cytoskeletal arrangement and cell contractility.
    • Fig. 2 Cell geometry regulates plasma membrane morphology and topography.
    • Fig. 3 Signal intensity of lipid raft markers is dependent on cell geometry.
    • Fig. 4 Akt recruitment to the plasma membrane and activation are dependent on cell geometry.
    • Fig. 5 Lipid rafts and Akt signalling mediate cell-geometry-dependent hMSC differentiation.

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系