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标题:Protective effect of ginsenoside Rg1 on LPS-induced apoptosis of lung epithelial cells.
时间:2020-01-15 20:55:40
DOI:10.1016/j.molimm.2018.11.003
PMID:30471963
作者:Qijian;Ji;Zhaozui
关键词:Acute;lung;injury;(ALI);Apoptosis;Autophagy;Ginsenoside;Rg1;Lipopolysaccharide;(LPS)
摘要:Sepsis-induced acute lung injury (ALI) is a life-threatening medical condition with high mortality and morbidity in the critical care units. Though, it was commonly accepted that inflammation and apoptosis of lung epithelial cells played an essential role in the pathogenesis of ALI, the underlying mechanism remain unknown. In our study, we found that LPS-induced cell apoptosis could be counteracted by elevated cell autophagy. In LPS-treated MLE-12 cells, suppression of autophagy via 3-MA could aggravate LPS-induced apoptosis, while activation of autophagy via Rapamycin could effectively impair the apoptosis of MLE-12 cells induced by LPS. In order to further discover the molecular regulation mechanism between apoptosis and autophagy in LPS-treated MLE-12 cells, we demonstrated that autophagy could induced the expression of Nrf2, followed with the decrease of p-p65. Targeted inhibition of Nrf2 could induce enlarged cell apoptosis via increasing the level of p-p65. In addition, we demonstrated that ginsenoside Rg1 protected MLE-12 cells from LPS-induced apoptosis via augmenting autophagy and inducing the expression of Nrf2. Our data implicates that activation of autophagy and Nrf2 by ginsenoside Rg1 may provide a preventive and therapeutic strategy for ALI.
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目录:
  • Protective effect of ginsenoside Rg1 on LPS-induced apoptosis of lung epithelial cells
    • Introduction
    • Materials and methods
      • Ethics statement
      • Cell culture and transfection
      • Acute lung injury mice model
      • Cell viability and growth inhibition test (CCK8)
      • Immunofluorescence
      • Western blot
      • RNA extraction and real-time PCR
    • Histology
      • Immunohistochemistry
      • Statistical analysis
    • Result
      • LPS induces apoptosis of MLE-12 cells
      • Induction of autophagy suppresses LPS-induced cell apoptosis via activating Nrf2
      • Ginsenoside Rg1 alleviated LPS-induced cell apoptosis through inducing cell autophagy
      • Ginsenoside Rg1 alleviated LPS-induced ALI
    • Discussion
    • Author contributions
    • Conflicts of interest
    • Acknowledgments
    • References

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