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标题:High-titer lactic acid production by Lactobacillus pentosus FL0421 from corn stover using fed-batch simultaneous saccharification and fermentation.
时间:2020-01-15 16:54:00
DOI:10.1016/j.biortech.2016.04.034
PMID:27128191
作者:Jinlong Hu;Yanxu Lin;Zhenting Zhang
关键词:Lactic acid fermentation;Lactobacillus pentosus;Simultaneous saccharification and fermentation;Corn stover
摘要:Because the cost of refined sugar substrate and limit of worldwide food availability, lignocellulosic materials are attractive for use in lactic acid (LA) production. In this study, we foundLactobacillus pentosusstrain FL0421 produced LA with high yields (0.52–0.82g/g stover) from five NaOH-pretreated and washed agro stovers through simultaneous saccharification and fermentation (SSF). We developed a fed-batch SSF process at 37°C and pH 6.0 using the cellulase of 30FPU/g stover and 10g/L yeast extract in a 5-L bioreactor to produce LA from 14% (w/w) NaOH-pretreated and washed corn stover under non-sterile condition. The LA-titer, yield and productivity reached 92.30g/L, 0.66g/g stover and 1.92g/L/h, respectively; and acetic acid titer and yield reached 34.27g/L and 0.24g/g stover. This study presented a feasible process for LA production from agro stovers and provided a candidate strain for genetic engineering for high-titer and -yield lignocellulosic LA production.
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目录:
  • High-titer lactic acid production by Lactobacillus pentosus FL0421 from corn stover using fed-batch simultaneous saccharification and fermentation
    • 1 Introduction
    • 2 Materials and methods
      • 2.1 Raw materials, enzymes, and medium
      • 2.2 Genomic characterization of Lb. pentosus FL0421
      • 2.3 Fermentation of pure sugars to produce LA in flasks
      • 2.4 SSF of LA from NaOH-pretreated lignocellulosic materials
        • 2.4.1 LA production by fermentation of different lignocellulosic materials
        • 2.4.2 Production of LA from corn stover in a 5-L bioreactor
      • 2.5 Analysis of sugars, acids, and inhibitors
    • 3 Results and discussion
      • 3.1 Identification of Lb. pentosus FL0421 and its fermentation features
      • 3.2 High-yield LA production by Lb. pentosus FL0421 from 5 lignocellulosic materials
      • 3.3 High-titer and high-yield LA production from NaOH-pretreated corn stover using fed-batch SSF under non-sterile conditions
    • 4 Conclusions
    • Acknowledgement
    • References

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