在线客服: 点击这里给我发消息  新用户使用步骤:会员注册→充值→重新登入→进入资源
标题:Gestational Hypoxia Induces White Matter Damage in Neonatal Rats: A New Model of Periventricular Leukomalacia
时间:2020-01-14 23:29:25
DOI:10.1111/j.1750-3639.2004.tb00492.x
作者:Olivier Baud;Jean-Luc Daire;Yvette Dalmaz
关键词:Brain;Animals;Animals, Newborn;Humans;Rats;Rats, Sprague-Dawley;Leukomalacia, Periventricular;Prenatal Exposure Delayed Effects;Disease Models, Animal;Anoxia
出版源: 《Brain Pathology》 ,2004 ,14 (1) :1-10
摘要:<p>In the premature infant, periventricular leukomalacia, usually related to hypoxic‐ischemic white matter damage, is the main cause of neurological impairment. We hypothesized that protracted prenatal hypoxia might induce white matter damage during the perinatal period. Pregnant Sparague‐Dawley rats were placed in a chamber supplied with hypoxic gas (10% O<sub>2</sub> ‐90% N<sub>2</sub>) from embryonic day 5(E5) to E20. Neonatal rat brains were investigated by histology, immunocytochemistry, western blotting, in situ hybridization, DNA fragmentation analysis, and in vivo magnetic resonance imaging (MRI).</p> <p>Body weight of pups subjected to prenatal hypoxia was 10 to 30% lower from p0 to P14 than in controls. Specific white matter cysts wear detected between p0 and p7 in pups subjected to prenatal hypoxia, in addition to abnormal extra‐cellular matrix, increased lipid peroxidation, white matter cell death detected by TUNEL and increased activated macrophage counts in white matter. Subsequently, gliotic scars and delayed myelination primarily involving immature oligodendrocytes were seen In vivo MRI with T1, T2, and diffusion sequences disclosed similar findings immediately after birth, showing strong correlations with histological abnormalities. We speculate that protracted prenatal hypoxia in rat induces abnormalities. We speculate that protracted prenatal hypoxia in rat induces white matter damage occurring through local inflammatory response and oxidative stress linked to re‐oxygenation during the perinatal period.</p>
大小:986 kb
页数:11 PAGES
下载: 点击下载
预览:

浏览器不支持嵌入PDF阅读,打开新页面在线阅读

本页内容由网络收集而来,版权归原创者所有,如有侵权请及时联系