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标题:Neuroprotective effect of tanshinone IIA against neuropathic pain in diabetic rats through the Nrf2/ARE and NF-κB signaling pathways.
时间:2020-01-14 22:34:48
DOI:10.1016/j.kjms.2018.03.005
PMID:30041760
作者:Feng Fa-Bo; Qiu Hai-Yan
出版源: 《Kaohsiung Journal of Medical Sciences》 :S1607551X17308471-
摘要:The study aims to investigate whether tanshinone (TSN) IIA affects diabetic neuropathic pain (DNP) via the Nrf2/AR and NF-κB signaling pathways. Rats were randomly assigned into DNP group, TSN group (injected with TSN IIA), TSN+DRB group (injected with TSN IIA and 15mg/kg Nrf2/ARE inhibitors), TSN+PDTC group (injected with TSN IIA and 60mg/kg NF-κB inhibitors) or control group. The first four groups were successfully established as DNP models after injection of streptozotocin. The blood glucose level, mechanical withdrawal threshold (MWT), thermal withdrawal latency (TWL), nerve conduction velocity (NCV) and antioxidase level were detected. Transmission electron microscopy and toluidine blue staining were utilized to observe the sciatic nerve. RT-qPCR and western blot were used to measure expression levels of Nrf2/ARE and NF-κB signaling pathway-related genes. Blood glucose, malondialdehyde (MDA) and erythrocyte glutathione peroxidase (GSH-Px) levels as well as expression of Keap1 and NF-κB were increased in the TSN, TSN+DRB and TSN+PDTC groups compared to control group. Furthermore, the MWT, TWL, NVC, and superoxide dismutase (SOD) levels and expression of Nrf2, heme oxygenase 1 (HO-1) and inhibitory kappa B (IκB) decreased in the treatment groups. The TSN+DRB and TSN+PDTC groups showed similar trend when compared with the TSN group, while the opposite trend was observed in the TSN group when compared with the DNP group. Our study demonstrates that TSN IIA alleviates neuropathic pain by activating the Nrf2/ARE signaling pathway and inhibiting the NF-κB signaling pathway in diabetic rats.
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目录:
  • Neuroprotective effect of tanshinone IIA against neuropathic pain in diabetic rats through the Nrf2/ARE and NF-κB signaling ...
    • Introduction
    • Materials and methods
      • Ethics statement
      • Model establishment and animal grouping
      • Blood glucose test
      • Behavioral test
      • Nerve conduction velocity (NCV) test
      • Transmission electron microscopy (TEM)
      • Toluidine blue staining
      • Coomassie brilliant blue staining
      • Reverse transcription quantitative polymerase chain reaction (RT-qPCR)
      • Western blot analysis
      • Statistical analysis
    • Results
      • Blood glucose levels at different time points in the control, DNP, TSN, TSN + DRB and TSN + PDTC groups
      • Mechanical allodynia and thermal hyperalgesia at different time points in the control, DNP, TSN, TSN + DRB and TSN + PDTC g ...
      • NCV at different time points in the control, DNP, TSN, TSN + DRB and TSN + PDTC groups
      • Structures of the sciatic nerve in the control, DNP, TSN, TSN + DRB and TSN + PDTC groups
      • Distribution of the sciatic nerve in the control, DNP, TSN, TSN + DRB and TSN + PDTC groups
      • Levels of antioxidase in the sciatic nerve among the control, DNP, TSN, TSN + DRB and TSN + PDTC groups
      • The mRNA and protein expression of Nrf2, HO-1, IκB, Keap1 and NF-κB in the control, DNP, TSN, TSN + DRB and TSN + PDTC groups
    • Discussion
    • Funding
    • Acknowledgements
    • References

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