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标题:A lysosome-targeted BODIPY as potential NIR photosensitizer for photodynamic therapy
时间:2020-01-14 21:38:31
DOI:10.1016/j.dyepig.2017.07.048
作者:Mingle;Li;Ruisong
关键词:BODIPY;Photosensitizer;Targeting;Lysosomes;Photodynamic;therapy
摘要:As an indispensable organelle, lysosomes play critical role in cell digestion and are closely relate to cell death and apoptosis. Photosensitizers that target to lysosomes are extremely important to photodynamic therapy. Herein, we report an excellent BODIPY based photosensitizer MBDP for lysosome targeting NIR photosensitizer. MBDP exhibits excellent PDT efficiency with low IC50 of 0.2 μM compared to the classical photosensitizer Ce6 (IC50 = 1.39 μM). Furthermore, MBDP can localize in lysosomes of cancer cells and produce significant singlet oxygen with photoexcitation of 660 nm red light. Additionally, lysosome destruction test and photoinduce cell death assay demonstrate that MBDP-mediated PDT is able to damage the lysosome integrity severely and result in cell apoptosis and death in lysosomal related pathway
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目录:
  • A lysosome-targeted BODIPY as potential NIR photosensitizer for photodynamic therapy
    • 1. Introduction
    • 2. Experimental
      • 2.1. Materials and general methods
      • 2.2. Synthesis
        • 2.2.1. Synthesis of compound 1
        • 2.2.2. Synthesis of compound 2
        • 2.2.3. Synthesis of compound 3
        • 2.2.4. Synthesis of MBDP
      • 2.3. Singlet oxygen quantum yield (ΦΔ)
      • 2.4. Cell incubation
      • 2.5. Subcellular localization
      • 2.6. Lysosomes disruption assay
      • 2.7. Intracellular singlet oxygen detection
      • 2.8. Confocal imaging of photoinduced cell death
      • 2.9. In vitro cytotoxicity assays
    • 3. Results and discussion
      • 3.1. Molecular preparation and basic spectroscopic properties
      • 3.2. Singlet oxygen generation and photostability
      • 3.3. In vitro photodynamic therapy evaluation
      • 3.4. Intracellular singlet oxygen detection
      • 3.5. Sub-cellular localization assays
      • 3.6. Lysosome disruption assays
      • 3.7. Confocal imaging of photoinduced cell death
    • 4. Conclusions
    • Acknowledgements
    • Appendix A. Supplementary data
    • References

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