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标题:Early intervention with faecal microbiota transplantation: an effective means to improve growth performance and the intestinal development of suckling piglets.
时间:2020-01-14 20:02:26
DOI:10.1017/s1751731118001611
PMID:29983136
作者:Cheng C. S.; Wei H. K.; Wang P.
出版源: 《Animal An International Journal of Animal Bios... :1-9
大小:461 kb
页数:10 PAGES
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目录:
  • Early intervention with faecal microbiota transplantation: an effective means to improve growth performance and the intestinal development of suckling piglets
    • Implications
    • Introduction
    • Materials and methods
      • Animals and housing
      • Preparation of faecal microbiota suspension of donor sows
      • Transplantation of faecal microbiota experiment and treatments
      • Determination of growth performance and diarrhea rate
      • Sample collection
      • Quantification of selected faecal bacteria
      • Faecal and plasma short-chain fatty acids analysis
    • Table 1Absolute quantitative real-time PCR Primers for detecting microbial populations in faeces of suckling piglets
      • Determination of hormones, zonulin, endotoxin, diamine oxidase, cytokines in plasma, and lipocalin-2 and secretory immunoglobulin A in faeces
      • Statistical analysis
    • Results
      • Growth performance and diarrhoea rate of piglets
      • Plasma concentrations of hormones
      • Selected microbial populations in faeces
      • Faecal and plasma short-chain fatty acids concentrations
    • Table 2Effect of faecal microbial transplantation on the growth „performance and diarrhea rate of suckling piglets1
    • Table 3Effect of faecal microbial transplantation on plasma „parameters of suckling piglets1
      • Plasma zonulin, endotoxin, diamine oxidase, and inflammatory cytokines and faecal lipocalin-2 measurement
      • Plasma immunoglobulins and faecal secretory immunoglobulin A concentrations
    • Figure 1Effect of faecal microbial transplantation (FMT) on seletcted microbial populations in faecal samples of 7-day-old piglets (a), „14-„day-old piglets (b) or 21-day-old piglets (c) Tot€=€total bacteria; Lacto€=€Lactobacillus spp.; Ente
    • Figure 2Effect of faecal microbial transplantation (FMT) on short-chain fatty acids (SCFAs) in faecal (a) and plasma (b) samples of suckling piglets on day 21.
    • Figure 3Effect of faecal microbial transplantation (FMT) on plasma zonulin (a), endotoxin (b) and diamine oxidase (c) and faecal lipocalin-2 (d) of suckling piglets.
      • Plasma inflammatory cytokines measurement
    • Discussion
    • Table 4Effect of faecal microbial transplantation on plasma „concentrations of cytokines in piglets1
    • Figure 4Effect of faecal microbial transplantation (FMT) on plasma immunoglobulin G (IgG) (a) and faecal secretory immunoglobulin A (sIgA) (b) of suckling piglets.
    • Acknowledgements
    • ACKNOWLEDGEMENTS
    • Supplementary material
    • References

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